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The insulin assay [17] mixture contained 1 mg/ml insulin and 10 M schistosome Trx1 [18], and enzyme activity was monitored by observing the decrease in A 340 during the first 3 min due to consumption of NADPH ( 340 nm = 6.22 mM 1 cm 1 )

These findings suggest that pubertal events, including male sexual function and spermatogenesis, appear to be primarily testosterone mediated

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