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To further investigate whether monotremes evolved a DPP-4 independent pathway to degrade GLP-1, we firstly confirmed the presence of DPP-4 in platypus and echidna sera using a synthetic DPP-4 peptide as a substrate and a DPP-4 inhibitor to prevent this cleavage (Supplementary Fig

Earlier mechanistic research confirmed that glutathione dose-dependently inhibits melanin synthesis by interrupting tyrosinase-L-DOPA interaction rather than directly inhibiting the tyrosinase enzyme itself

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