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During treatment, daily fluid intake must be 2000 ml, and urine alkalization is required to prevent kidney stones

As can be seen from Volcano Figure 6 , the FI group up-regulated 6 differential metabolites and down-regulated 12 differential metabolites relative to the NC group

(4) Centrifuge for 10 min (12000 rpm, 4C), aspirate 150 L of the supernatant with a syringe, filter it using a 0.22 m organic phase pinhole filter, transfer it to an LC injection vial and store it at -80C until LC-MS analysis

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Printable cheat sheet Keep a condensed U-100 reference handy for print or quick bench-side review