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Blots were treated with primary antibodies, including anti-lysyl oxidase-like 1 (LOXL1) (1:1000, ab313585, Abcam), anti-periostin (Postn) (1:500, sc-398631, Santa-Cruz), anti-interferon stimulated gene 15 (ISG15) (1:1000, 2743, Cell Signaling Technology), anti-superoxide dismutase-3 (Sod3) (1:1000, ab80946, Abcam), anti-peroxiredoxin (Prdx) 4 (1:1000, ab184167, Abcam), anti-aldehyde dehydrogenase 1a1 (ALDH1a1) (1:1000, ab52492, Abcam), anti-Prdx6 (1:1000, ab133348, Abcam), anti-glutathione S-transferase M1 (GSTM1) (1:1000, PA5-79337, Invitrogen), and anti--actin (1:1000, 4970, Cell Signaling Technology), at 4 C overnight, and IRDye 800 CW-conjugated secondary antibodies for 2 h at room temperature, subsequently
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Excess copper promotes glutathione (GSH) oxidation, leading to catecholamine oxidation (39)
3,47 Animal models also suggest that lead can be absorbed through the skin
However, these chemotherapeutic agents generally suffer from a lack of selectivity and optimal pharmacokinetics, and their broad mechanisms of action often lead to severe systemic toxicity 3
DM: Writing original draft, Methodology, Formal analysis, Writing review & editing
