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The beads were packed into a gravity column, and washed with 15 CV of buffer with 10 mM imidazole in buffer 0.03%(w/v) DDM, 0.006%(w/v) CHS, 1 M NaCl, 4%(w/v) glycerol, 0.05 mM EDTA, 0.2 mM TCEP, 0.1 protease inhibitor cocktail, 20 mM HEPES pH 7.5

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& Gassmann, M

Plant J 12:179190 Brczi A, Mller IM (1998) NADH-monodehydroascorbate oxidoreductase is one of the redox enzymes in spinach leaf plasma membranes

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Secondary endpoints included changes in total alcohol intake, number of drinks per day, number of days without alcohol intake, alcohol craving, plasma enzymes, body weight, hemoglobin A1c (HbA1c), waist circumference, and self-reported measures of quality of life and health, among others