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Each sample was homogenized using a TissueLyser III (Qiagen) with 5 homogenization beads in 200 l of cold Tris lysis buffer (150 mM NaCl, 20 mM Tris, pH 7.4, 1 mM EDTA, 1 mM EGTA, 1% Triton X) containing a cocktail of protease (cOmplete Protease Inhibitor Cocktail, Roche) and phosphatase inhibitors (Phosphatase Inhibitor Cocktail 2 and 3, Sigma Aldrich), NaF and PMSF in DMSO

You typically administer a measured dose via the nostrils, often shortly before your bedtime routine

In saline-treated rats, Dox exposure caused sarcolemmal vacuolization, myofibrillar fragmentation, and extensive mitochondrial injury, including swelling, cristae disruption, and membrane dilation

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Manufactured in an NSF-certified facility following GMP standards

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