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Antibody to NeuN is specific to the nucleus and cytoplasm of neurons only, and our results clearly provide the evidence that iNOS was indeed perfectly colocalized with NeuN, as shown in Figs

This modification is catalyzed by an enzymatic cascade consisting sequentially of the NEDD8-activating enzyme E1, NEDD8-conjugating enzyme E2, and substrate-specific NEDD8-E3 ligase (66)