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Cell transfection For transfection, pCDNA3.1-LRP1-1(amino acids 1-1182)-Flag, pCDNA3.1-LRP1-2(amino acids 11832473)-Flag, pCDNA3.1-LRP1-3(amino acids 24743284)-Flag, pCDNA3.1-LRP1-4(amino acids 32853872)-Flag or pCDNA3.1-LRP1-5(amino acids 38734544)-Flag vectors were transfected into HEK293T cells using polyethyleneimines

[DOI] [PubMed] [Google Scholar] 53.Hassan SZ, Gheita TA, Kenawy SA, Fahim AT, El-Sorougy IM, Abdou MS

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These simulations indicate that the ECD in the apo-state GLP-1R is quite dynamic and that the TMD-interacting closed conformations are energetically favorable in the absence of the peptide agonist

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doi.org/10.3390/ijms19071987 Seiwerth, S., et al