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After harvesting, cells were plated to a density of 6 0.5 10 5 cells/mL in six-well plates (Corning) and allowed to incubate for 72 h at 37C in a 5% (v/v) CO 2 atmosphere to a confluence of 90%

We then knocked down hmr-1 in the DTC of glp-1 mutants to confirm whether it controls gonadal longevity through the DTC-GSC adhesions

The B vitamin family has thiamine (B1), riboflavin (B2), niacin (B3), pantothenic acid (B5), pyridoxine (B6), biotin (B7), folate (B9), and cobalamin (B12)

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Drug Targets 8 , 347357 (2007)

Only studies in English were included in the meta-analysis