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If the HCD fragmentation had the fragment ion-184.0733 m/z (phosphocholine head group), then the same ions were subjected either to ddMS2 CID (fixed collision energy-32%, activation time-10 ms & Q-0.25, 30K resolution, 100 % normalized AGC target and maxIT-54 ms) or CID MS3 scans (fixed collision energy-35%, activation Q-0.25) triggered on the top 3 most intense ions that lost neutral fatty acids plus ammonia (only for triacylglycerols)

10.1146/annurev-biochem-013118-111429

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doi: 10.1101/pdb.prot095505 21 LoverA

This timing is important as it: Helps rehydrate the body after a nights sleep Provides essential minerals to kickstart your day Taking it in the morning supports hydration and prepares your body for the day ahead