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Copper (II) sulfate pentahydrate, iron (III) chloride hexahydrate, ascorbic acid, disodium ethylenediaminetetraacetate dihydrate (EDTA), 5,5-dithiobis-2-nitrobenzoicacid (DTNB), tris(hydroxymethyl)aminomethane (TE8), cysteine, -L-glutamyl-L-cysteine and glutathione were purchased from Sigma Aldrich (St Louis, USA)

Alpha Lipoic Acid is a fat and water-soluble antioxidant that also boosts glutathione levels

The mobility of the population as well as the inability to precisely estimate exposure to ozone and other potential confounders over a period of many years degrades the power of, and leads to bias in, both longitudinal and cross-sectional studies

& Sukhatme, V

P.StrassburgA.KneipS.BarutA.TukeyR

Subsequently, the cells were lysed using 1 lysis buffer (Beyotime, China) containing protease inhibitor cocktail 2 (Roche, Switzerland) directly in the plates to obtain whole-cell lysates