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Absolute losses approached 26 kg in some cases, often accompanied by significant reductions in fat mass and waist circumference while showing relative preservation of lean mass in various analyses

The slides were again immersed in PBS, washed three times in PBS, and then counterstained in the dark with DAPI solution for 10 min at room temperature

It should be handled only by qualified personnel in an appropriate laboratory setting and stored according to standard practices for research chemicals

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