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All analyses were performed following the manufacturers recommendations

To further validate the altered mitochondrial dynamics induced by palmitoyl-L-carnitine, SH-SY5Y cells were transfected with a GFP-Mito plasmid after treatment with BSA-PC or BSA alone

The Griess reaction was based on a diazotization reaction in which acidified nitrite reacted with diazonium ions and, in a further step, was coupled to N-1-naphthyl ethylenediamine dihydrochloride, forming a chromophoric azo derivate

The starting dose of 2.5 mg is maintained for four weeks, followed by 5 mg for four weeks, then 7.5 mg, 10 mg, 12.5 mg, and finally 15 mg

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