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For the LEfSe analysis, an alpha of 0.05 was set for the KruskalWallis tests among classes, and per-sample normalization was performed to bring the sum of the values to 1 M

References Heffernan M, Summers RJ, Thorburn A, Ogru E, Gianello R, Jiang WJ, et al

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They are not for human consumption and are not intended to diagnose, treat, cure, or prevent plantar fasciitis, chronic foot pain, sports injuries, soft tissue injury, inflammation, pain, stiffness, reduced mobility, recovery delay, performance decline, or any medical condition

Per the FY2026 ICD-10-CM Official Guidelines Section I.C.14, the CKD stage code is sequenced first