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Calculating your working concentration Use this formula: Concentration (mcg/mL) = Total peptide (mcg) Volume of diluent added (mL) | Vial size | Bacteriostatic water added | Resulting concentration | |-----------|---------------------------|------------------------| | 2 mg (2,000 mcg) | 1 mL | 2,000 mcg/mL | | 2 mg (2,000 mcg) | 2 mL | 1,000 mcg/mL | | 6 mg (6,000 mcg) | 3 mL | 2,000 mcg/mL | | 6 mg (6,000 mcg) | 6 mL | 1,000 mcg/mL | Most prescribers prefer the 1,000 mcg/mL concentration because the injection volume for a 200 to 500 mcg dose falls between 0.2 mL and 0.5 mL

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