Hippocampal neurons were maintained in Neurobasal A media from Invitrogen (Carlsbad, CA) supplemented with B27 from Invitrogen 0.5 raM L-glutamine, and 5mM cytosine-D-arabinofuranoside from Sigma added at 2 days in vitro
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Short- and medium-term plasticity associated with augmenting responses in cortical slabs and spindles in intact cortex of cats in vivo
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New experience activates a cascade of events in neurons whereby each step triggers specific molecular mechanisms necessary for the full expression of long-term synaptic plasticity