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Proteins (1030 g/well, equal amounts within group but different amounts among groups for better comparison) were separated on 10% or 12% SDS-PAGE gel and transferred on an equilibrated polyvinylidene difluoride membrane (PVDF, Amersham Biosciences, Buckinghamshire, UK)

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The method was validated by visually identifying the nuclei and scoring the cell number using wild-type germlines

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2016;11(9):e0162998

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The preservative may lose effectiveness

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This duality highlights the importance of cell type, disease stage, and metabolic state when interpreting lactate-related findings

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Regular or diet

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