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It is possible that additional satiety signals, such as glutamate 40 , GABA 41 , cholecystokinin (CCK) 42 , pro-opiomelanocortin (POMC) 43 , neuropeptide Y 44 , and/or norepinephrine 44 , are mediating the macronutrient-dependent feeding responses associated with NTS neuron activation

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This fragment was digested with EcoRI (NEB) and ligated into pGex-6p1 (GE Healthcare) prepared by digestion with BamHI and blunting with Mung Bean Nuclease (NEB) to generate pJB-GST-HTS