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Kostenuik PJ, Harris J, Halloran BP, Turner RT, Morey-Holton ER, Bikle DD

Following cleavage with Precision Protease (Beyotime) to remove the GST tag, the eluted proteins underwent further purification by size-exclusion chromatography on a Superdex 200 10/300 GL column (GE Healthcare) using an elution buffer of 50 mM HEPES (pH 7.4) and 150 mM NaCl

For those new to IV therapy, this is often the best starting point because it provides a broad spectrum of support for general wellness and a gentle energy boost

This type of anemia is caused by a lack of the intrinsic factor needed to support red blood cell production

Cagrilintide was initiated at 0.3 mg for the 0.3 mg group, or 0.6 mg for all higher-dose groups and escalated every two weeks until reaching the final dose