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Thymosins in multiple sclerosis and its experimental models: moving from basic to clinical application
Immunoblotting Cell pellets were washed twice with ice-cold PBS prior to lysis in RIPA buffer (10 mM Tris-Cl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% SDS) supplemented with protease inhibitors Sigma-Aldrich, 11836170001) and phosphatase inhibitors (Roche, 04906837001)
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Additionally, we tested whether the time between sample collection and deproteinization impacted plasma GSH in its various forms (total GSH, free GSH, GSSG, and the ratio of free GSH to GSSG) in heparinized and EDTA-treated plasma samples
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cocaethylene Notes: One of the most addictive drugs known (Schedule II)
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