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DHFR: dihydrofolate reductase

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They are distinct pharmacological entities and are not interchangeable in an experiment

Single- or double-antigen labeling of formalin-fixed and paraffin-embedded 4-m-thick sections by immunocytochemistry (ICC) or immunohistochemistry (IHC) included the following steps: deparaffinization, rehydratation, boiling in EDTA buffer (1 mM EDTA, 0.05% Tween 20, pH 8.0) for 15 min, 1% H 2 O 2 incubation for 15 min and blocking in SuperBlock solution (ScyTek Laboratories, Logan, UT) for 30 min, followed by primary antibody application

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doi: 10.1016/j.tmrv.2003.10.003 12 KononovSUMeyerJFrahmJKerstenSKluessJMeyerUet al