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This result was confirmed in a study on the mouse colon, where only GPX1 and GPX3 RNA decreased, while the RNA of GPX2 and GPX4 remained stable when mice were deficient in selenium (Kipp, 2017)

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8 mM of reducing agent DTT (Sigma) was then added to the samples and incubated in a thermomixer at 30C for 30 minutes, followed by the addition of 20 mM iodoacetamide (Sigma) for protein carboxylation with incubation at 30C in the dark for 30 minutes

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Crucially, despite this variability, subsequent meta-analyses pooling the data from injectable GLP-1RAs have demonstrated a significant overall reduction in the risk of hospitalisation for HF with GLP-1RAs