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His 6 -tagged p97 constructs were purified 30 using Ni 2+ -NTA affinity chromatography followed by TEV protease cleavage, followed by size-exclusion chromatography (SEC) on a Superdex 200 column (Cytiva)

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Essa propriedade despertou grande interesse porque o cobre um micronutriente essencial para inmeras reaes bioqumicas, atuando como cofator de enzimas envolvidas na defesa antioxidante, na produo de energia celular e na organizao da matriz extracelular

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