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Primary antibodies includes rabbit anti-phospho-H3 (1:100, Cell signaling, cat#9701), rabbit anti-cleaved caspase 3 (1:100, Cell signaling, cat#9661), sheep anti-acetaminophen primary antibody (1:250, Bio-Rad, cat#0016-0104)

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Several possible reasons for the lack of efficacy of antioxidants in clinical trials include (i) insufficient antioxidant levels in the target organ, especially in the nervous system, because an integrated bloodbrain barrier (BBB) is a major obstacle to delivering desired substances, including antioxidants, into the CNS