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After five minutes, initiate the reaction by adding 20 L of the Cumene Hydroperoxide Solution into each well and mix thoroughly (it is recommended to use a multi-channel pipette)

NICE Clinical Knowledge Summaries and British Society for Haematology guidelines support B12 supplementation in these clinical contexts, with treatment regimens tailored to the underlying cause

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It is the most common cause of severe vitamin B12 deficiency in the UK and mainly affects those over 70 years of age