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The cell lysate was clarified at 13,400 g for 10 min at 4 C and the supernatant was subjected to a Cu(I)-assisted click reaction with biotin picolyl azide followed by loading onto a 10 kDa spin column to remove the free biotin picolyl azide using lysis buffer

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Another peptide, GHK-Cu, is being studied for potential cosmetic benefits such as for photoaging and hair loss and was added to the Category 1 list (but not for injectable routes of administration)