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Roderick JE, Hermance N, Zelic M, Simmons MJ, Polykratis A, Pasparakis M, et al

The Arg-280 residue stably interacts with guanine bases in the major groove region of the target DNA, whereas the interactions involving Lys-120, Cys-277, and Ala-276 are sequence specific

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Recombinant His-tagged AtGrxS15, AtGrxC1 and roGFP2 were also expressed and purified according to established protocols 34,36