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J Clin Oncol 19 : 21892200 Baserga R (1994) Oncogenes and the strategy of growth factors

Secondary antibodies (AlexaFluor 594 anti-mouse 1:500 (Invitrogen, A11005) and AlexaFluor 488 anti-rabbit 1:500 (Invitrogen, A11034)) were added to blocking buffer and incubated at room temperature for 1 h

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Subsequently, the mice received an intraperitoneal injection of 4-OHT at a dosage of 50 mg/kg in a final concentration of 10 mg/ml and were exposed to 2MT for an additional 1.5 h

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Persistent or severe hypoglycemic episodes despite proper meal timing