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Determination of Ergothioneine reactivity with H 2 O 2 Reactivity between EGT and H 2 O 2 was analysed by incubating 3 mM EGT (Sigma-Aldrich) with, and without, 3 mM H 2 O 2 for 3 h at room temperature

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Genetically encoded biosensors such as roGFP2-Grx1 allow real-time, reversible monitoring of the glutathione redox potential inside living cells without requiring lysis or fixation

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