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doi:10.3389/fphar.2014.00151 Mytilineou C1, Kramer BC, Yabut JA

As both 71 and 139 M NADPH concentrations were in excess relative to SmTGR, these samples were over reduced, show no evidence of flavin reoxidation, and could be fit to two exponentials

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Lysates were cleared by centrifugation at 12,000 g for 15 min at 4 C and the supernatants were stored at 80 C until analysis