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Cultures harvested by trypsinization were plated at a density of 40,00050,000 cells per well on rat tail collagen (RTC) coated 96-well Costar 3904 plates two days prior to each experiment

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Figure 3c shows that surface-targeted LaccID enriches N-cadherin but not calreticulin, while surface-targeted APEX2 and HRP enrich both markers because of (1) substantial pools of APEX2 and HRP in the ER lumen because of their imperfect targeting to the cell surface, and (2) high activity of APEX2 and HRP in the ER compartment