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however, neoantigen-specific CD 4 and CD 8 T cell responses were identified in three out of four patients, mostly following in vitro stimulation (IVS) of PBMCs

References 1.Maderson P.F.A

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Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark

Last updated on Jul 19, 2025