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Determination of Reactive Oxygen Species Levels The intracellular levels of reactive oxygen species were determined by staining the cells with CM-H 2 DCFDA (5-(and 6-) chloromethyl-2,7-dichlorodihydrofluorescein diacetate, acetyl ester) (Molecular Probes, Eugene, Ore, USA) in PBS as previously described in [26]

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