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For recruitment assays, INS-1 832/3 GLP-1R KO cells were seeded onto 6-well plates and transiently transfected with a combination of 1 g GLP-1R-SmBiT WT or V229A and either 1 g of LgBiT-mini-G s , -mini-G q or --arrestin 2

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This pentadecapeptide makes up a fraction of the entire BPC protein, with 15 amino acids

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