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The model was improved through iterative cycles of manual model building with Coot and refinement with REFMAC in the CCP4 suite 48,49

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Some clinical trials have used 1 mg per day , although higher doses do not necessarily produce better results

Denninger JW, Marletta MA (1999) Guanylate cyclase and the NO/cGMP signaling pathway

Vitamin B12 is naturally found in animal products

The people at risk of getting low levels of B12 are: Vegans and vegetarians as its not in their diet Over 60s as they have reduced absorption within the gut Anti acid medication or metformin Chronic digestive disorders such as Crohns, celiac disease or IBS Eating disorders or people who have had weight loss surgery Drink alcohol or abuse nitrous oxide People who are HIV positive There are different types of synthetic vitamin B12