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Dogs over 110 lbs, start at 2 mL daily for a month, recheck B12 levels, and taper based on serum levels

Traditional treatments are more widely available and some countries have subsidies, so theyre more accessible to the majority

Twenty-four hours later, medium was replaced with 200 L of the Seahorse assay medium [pyruvate free DMEM (US Biological, D9802) supplemented with 10% dialyzed FBS and 25 mM HEPES-KOH with pH adjusted to 7.4]

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