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Nausea from tirzepatide is mediated by GLP-1 receptor activation in the area postrema (brainstem vomiting center) and peaks 2472 hours post-injection, whereas 5-amino-1MQ has no known direct emetic mechanism

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Fragments were dispensed in triplicate into a 384-well plate using an Echo 550 liquid handler to give a final ligand concentration of 250 M (total sample volume of 40 L)

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For comparison, our guide on how to reconstitute semaglutide covers the parallel process for the GLP-1-only compound