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202 YangJ.Sun-WaterhouseD.XieJ.WangL.ChenH.CuiC.et al (2018)

From a mechanical standpoint, rapid weight loss leads to: Reduced joint loading at the knees and hips

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The sample was centrifuged and resuspended in 1 ml of ice-cold 1 PBS buffer (pH 7.4)

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2.4.2 Amino acid analysis Amino acids were analysed using the ChromSystems MassChrom Amino Acid Analysis kit following the manufacturers instructions, which facilitates the quantification of 52 amino acids and creatinine in urine