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Antic T, Hyjek EM, Taxy JB

Sections were subjected to antigen retrieval using citrate buffer (0.1 M, pH 6.0) in a microwave for 7 min and endogenous peroxidase blocking using 3% H 2 O 2 in PBS for 30 min

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The resulting increase in Treg abundance dampens metaflammation and reduces circulating and islet levels of TNF-, IL-1, and IFN-, which in turn alleviates -cell ER stress and protects against cytokine-induced apoptosis (Kaisar et al., 2017)