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Cells were washed three times in 500 l PBS, lysed with 0.2% Triton X-100 at 37 C for 10 min and plated on LB agar

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The near-balanced peptide was shown to be ~2-fold less potent at GLP-1R and ~10 fold less potent at GCGR than native ligands in cAMP synthesis (75)

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2013;36(Suppl 1):S11S66