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The vector for protein production, pJB-GST-HTS-HS-GFP, was created by first cloning a 7 amino acid spacer fragment containing an XhoI site into pET-15b (Novagen) using sticky ligation and BamHI/XhoI sites

Nitric oxide is a critical signaling molecule that regulates blood flow, inflammation, immune function, and cellular communication throughout the body

Two separate phases were observed at an excitation of 484 nm, a rapid but small increase and a slower but more pronounced decrease of fluorescence (Fig

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A subset of cultured cells expressed GLP- 1 receptor, and mice with a-cell specific GLP- 1 receptor knockout have mild glucose intolerance and higher glucagon secretion in response to glucose challenges than animals of the wild type, which may indicate that GLP- 1 directly affects this cell [69]

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