The 2017 Neelakantan paper in Biochemistry characterised a fluorescent assay for direct real-time monitoring of NNMT enzymatic activity, providing the analytical foundation for inhibitor screening including 5-Amino-1MQ (PMID 28121423)
severe thiamine deficiency, while rare, can cause Wernickes encephalopathy in hyperemesis gravidarum, and inadequate B is associated with anemia and neurologic issues in the newborn
Requirements of vitamin B12 in excess of normal (due to pregnancy, thyrotoxicosis, hemolytic anemia, hemorrhage, malignancy, hepatic and renal disease) can usually be met with oral supplementation
GHK-Cu: collagen and structure GHK-Cu is a copper-binding tripeptide associated with regulation across more than 4,000 genes, including collagen synthesis, antioxidant defense, wound healing, inflammation, and tissue breakdown. Circulating levels decline with age, from roughly 200 ng/mL at age 20 to about 80 ng/mL by 60. It clears damaged matrix while helping new collagen form and cross-link into stronger structure (matrix remodeling and lysyl oxidase activation). BPC-157: blood supply BPC-157 is a 15-amino-acid fragment from gastric juice
A typical dosage regimen starts with higher amounts and gradually tapers off
Vitamin B12 as a treatment for peripheral neuropathic pain (Julian et al., 2020) systematic review
