Quantitative real-time polymerase chain reaction (qRTPCR) To determine mRNA expression, total RNA was extracted from each sample group using TRIzol reagent, and cDNA synthesis was performed using a Transcription First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland)
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The above notion could reconcile the paradox of EBV exposure being a strict requirement for subsequent development of MS, and on the other hand the recent failure of an EBV-specific T-cell therapy (Giovannoni et al., 2024): if early EBV infection triggers a molecular cascade driving MS pathogenesis that no longer requires replication of virus, anti-viral therapies begun once MS is clinically evident may be doomed to failure
It is important to continue taking the medication as prescribed, even if you do not notice immediate improvement
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