Mitochondria were isolated (Miltenyi Biotec 130-096-946) from 10 10 6 pooled cells from 2 to 3 mice following the manufacturers protocol
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The Vmax signifies the peak rate of the enzymes kinetic reaction, which occurs when the substrates active site reaches saturation ( m represents the ease with which a substrate can saturate the enzyme
Antibodies against cardiolipin were also found in 92-95% of ME/CFS patients (509, 510), although they were only 4% in another study (506)
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