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For calcium-dependent aggregation assays, Syn WT (50 M) was pre-incubated with 3.0 mM CaCl for 30 min prior to initiating aggregation, as previously described 102

Patients should use a validated sedation scale (such as the Ramsay Sedation Scale or a simple self-reported drowsiness log) during the first 4 weeks of combination therapy

It is important to avoid putting unnecessary pressure on the wrist during this time

2.3 Experimental protocol Following 1 week of acclimation, six rats were allocated randomly to one of four groups: Rats in Group 1, the control group, were given saline intraperitoneally (IP) twice weekly for 6 weeks

These include age, poor nutrition, environmental pollutants, and high levels of stress

Do not apply any pressure at the injection site