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The tissue was finely minced using scissors and then digested in KRPH buffer (20 mM HEPES,5 mM KH2PO4,1 mM MgSO4.7H2),1mMCaCl2.2H2O,136 mM NaCl,4.7 mM KCL supplemented with 1 mg/ml collagenase A (Sigma-Aldrich) for 1 h at 37 C with gentle agitation

doi: 10.18637/jss.v082.i13 [DOI] [Google Scholar] 44.Kalra S, Kalra B, Sharma A

For centuries, it has been prescribed not to target a single ailment, but to build holistic resilience against physical and mental stress (Bonilla et al

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Laboratory and imaging assessments were limited, and improvements were primarily based on clinical observation and patient/caregiver reports