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The membranes were incubated with primary antibody (mouse anti-GLP-1R, sc-390774, Santa Cruz Biotechnology, Santa Cruz, CA, 1:1000 and rabbit anti-GAPDH, sc-25778, Santa Cruz Biotechnology, Santa Cruz, CA, 1:1000) in TBST overnight at 4 C

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Then, the cells were treated with low (5.5 mM) or high (30 mM) glucose concentrations with or without GSH (4 mg/mL) for 72 h

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