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The protein concentration of the resulting lysates was determined using the Quick Start Bradford Protein Assay Kit 2 (#5000202, Bio-Rad, USA)

In particular, in experiments with T2 and the Tn5 library constructed with the wecA strain of ECOR21 (which was generally more susceptible to infection by T2 and other phages), MOI was titrated in three parallel infection screens (with varying amounts of phage lysate added: 350 l, 100 l, and 25 l) to empirically ensure specific depletion of only the phage-sensitive mutants

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