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The renal or extrarenal expression of 1--hydroxylase (CYP27B1) enzymatic action converts 25(OH)VD 3 to an active metabolite 1,25-dihydroxy vitamin D 3 (1,25(OH) 2 VD 3) 13

The alcohol concentration in serum showed a decrease in the GSH group compared to the placebo group, but the difference was not statistically significant, and the AUC and C max were not statistically different

Sample Loading and First Incubation: Add 10 L of each test sample into the designated wells in duplicate

Finally, all slides were incubated with 3,3-diaminobenzidine tetrahydrochloride (Maxim, China) for 6 min and counterstained with hematoxylin

If any product remains, it should be stored closed in the refrigerator anduses one week after open

doi: 10.1016/j.jim.2011.01.015 23 JakemanG