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In addition, the insufficient activity of both enzymes was found to render Brown Norway rats susceptible to spontaneous artery rupture, which further indicated the pivotal role of LOXs in ECM regulation, especially in stabilizing collagen and elastin crosslinks (14,15)

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If you have an MTHFR mutation, supplementing with vitamin B12 may be beneficial, as these mutations can impair your body's ability to utilize B12 effectively, potentially leading to deficiency symptoms and elevated homocysteine levels

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Briefly, HaCaT keratinocytes were seeded at a density of 10,000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment

The Scientific World Journal , 2013